Contamination

Last week, our proteomic group did a 2D of a purified protein but, unfortunately, it seemed we had a contamination. So, this week-end, we performed blank tests in order to see if the contamination came from our experiment (or from the purification). We tested our fluorescent markers and different 1D strip holders. At a normal gain, the image looks fine: contamination doesn’t come from the 2D But when I increase the gain, the image (and thus our gel) is really contaminated! :-( We really have to look where did the contamination happen! Look at this: ...